Proteomics of human postnatal lung epithelial, endothelial, mesenchymal, and immune cells

Mereena G. Ushakumary¹, Song Feng¹, Gautam Bandyopadhyay², Heather Olson¹, Karl K. Weitz¹, Heidi L. Huyck², Cory Poole², Jeffrey M. Purkerson², Soumyaroop Bhattacharya², Cecilia Ljungberg³, Thomas J. Mariani², Gail H. Deutsch⁴, Ravi Misra², James P. Carson⁵, Joshua N. Adkins¹, Charles Ansong¹, Gloria Pryhuber², Geremy Clair¹

¹Pacific Northwest National Laboratory, ²University of Rochester Medical Center, ³Baylor College of Medicine, ⁴University of Washington, ⁵University of Texas at Austin, ^*Corresponding author

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Description The lung is a vital organ that undergoes extensive morphological and functional changes during postnatal development. To disambiguate how different cell populations contribute to organ development, we performed proteomic and transcriptomic analyses of four sorted cell populations from the lung of human subjects aged 0 to 8 years-old with a focus on early life. The cell populations analyzed included epithelial, endothelial, mesenchymal, and immune cells. Our results revealed distinct molecular signatures for each of the sorted cell populations that enable the description of molecular shifts occurring in these populations during post-natal development. We confirmed that the proteome of the different cell populations was distinct regardless of age and identified functions specific to each population. We identified a series of cell population protein markers, including those located at the cell surface, that show differential expression and distribution on RNA in situ hybridization and immunofluorescence imaging. We validated the spatial distribution of AT1 and endothelial cell surface markers. Temporal analyses of the proteomes of the four populations revealed processes modulated during postnatal development and clarified the findings obtained from whole tissue proteome studies. Finally, the proteome was compared to a transcriptomics survey performed on the same lung samples to evaluate processes under post-transcriptional control.

Dataset ID LMEX0000004404

Assay Type Proteomics

Organism Human

Stages Neonate | Infant | Child

Sample Count 44

Sample Prep Lmdata:sample_type_sorted_cells

Technology Mass Spectrometry

Reference Ushakumary, et al. (2024)

External databases MassIVE: MSV000094189

File	Description	Size
log2-normalized-MS.xlsx

LungMAP ID	Alt ID	Species	Sex	Race	Age	Age Group	GA at Birth	CGA	Weight	Weight Percentile	Cause of Death	Type of Death
LMSP0000000131	D008	Homo sapiens	Female	White	20 months	Child			12.25 kg	86	Trauma - Auto Accident	DBD (after brain death)
LMSP0000000134	D011	Homo sapiens	Female	White	20 months	Child	40 weeks		10.89 kg	53	Anoxic/Hypoxic Brain Injury - Drowning	DBD (after brain death)
LMSP0000000141	D018	Homo sapiens	Female	White	3 years	Child			15 kg	73	Anoxic/Hypoxic Brain Injury - Drowning	DBD (after brain death)
LMSP0000000142	D019	Homo sapiens	Male	White	1 days	Neonate	37 weeks, 3 days	37.43 weeks			Congenital Anomaly - Brain	DCD (circulatory death)
LMSP0000000145	D022	Homo sapiens	Male	Other	2 years	Child			11.79 kg	12	Trauma - Isolated Head Trauma	DBD (after brain death)
LMSP0000000035	D024	Homo sapiens	Male	Other	4 months	Infant			8.62 kg	94	Trauma - Auto Accident	DBD (after brain death)
LMSP0000000157	D036	Homo sapiens	Male	White	8 years	Child			34 kg	89	Trauma - Isolated Head Trauma	DBD (after brain death)
LMSP0000000159	D038	Homo sapiens	Female	Unknown or Not Reported	1 days	Neonate	39 weeks, 3 days	39.57 weeks	2.27 kg	1.2	Congenital Anomaly - Brain	DCD (circulatory death)
LMSP0000000162	D041	Homo sapiens	Male	White	6 days	Neonate	38 weeks, 3 days	39.14 weeks	3.24 kg	33	Anoxic/Hypoxic Brain Injury - Other	DCD (circulatory death)
LMSP0000000164	D043	Homo sapiens	Male	White	21 months	Child			9.8 kg	6.2	Trauma - Isolated Head Trauma	DBD (after brain death)
LMSP0000000165	D044	Homo sapiens	Male	White	6 days	Neonate	40 weeks, 3 days	41.57 weeks	3.63 kg	56	Anoxic/Hypoxic Brain Injury - Neonatal HIE	DCD (circulatory death)

11 of this study's 11 samples were also assayed by 317 other LungMAP studies, across 9 modalities. Click any node to list those studies.

Shared samples by study