Proteomic Analysis of Human Lung Development
1Pacific Northwest National Laboratory, 2University of Rochester Medical Center, *Corresponding author
Description
Rationale: The current understanding of human lung development derives mostly from animal studies. Although transcript-level studies have analyzed human donor tissue to identify genes expressed during normal human lung development, protein-level analysis that would enable the generation of new hypotheses on the processes involved in pulmonary development are lacking. Objectives: To define the temporal dynamic of protein expression during human lung development. Methods: We performed proteomics analysis of human lungs at 10 distinct times from birth to 8 years to identify the molecular networks mediating postnatal lung maturation. Measurements and Main Results: We identified 8,938 proteins providing a comprehensive view of the developing human lung proteome. The analysis of the data supports the existence of distinct molecular substages of alveolar development and predicted the age of independent human lung samples, and extensive remodeling of the lung proteome occurred during postnatal development. Evidence of post-transcriptional control was identified in early postnatal development. An extensive extracellular matrix remodeling was supported by changes in the proteome during alveologenesis. The concept of maturation of the immune system as an inherent part of normal lung development was substantiated by flow cytometry and transcriptomics. Conclusions: This study provides the first in-depth characterization of the human lung proteome during development, providing a unique proteomic resource freely accessible at Lungmap.net. The data support the extensive remodeling of the lung proteome during development, the existence of molecular substages of alveologenesis, and evidence of post-transcriptional control in early postnatal development.
Dataset ID
LMEX0000004395
Assay Type
Proteomics
Organism
Human
Stages
Neonate | Infant | Child
Sample Count
11
Sample Prep
Lmdata:sample_type_biopsy
Reference
Clair et al. (2022) | Press Release
External databases
Proteome Xchange: PXD020798
| File | Description | Size |
|---|---|---|
| Clair et al. (2022) Supplement | 29.1 MB |
| LungMAP ID | Alt ID | Species | Sex | Race | Age | Age Group | GA at Birth | CGA | Weight | Weight Percentile | Cause of Death | Type of Death |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LMSP0000000128 | D004 | Homo sapiens | Male | White | 5 days | Neonate | 41 weeks | 41.71 weeks | 3.63 kg | 63 | Anoxic/Hypoxic Brain Injury - Neonatal HIE | DBD (after brain death) |
| LMSP0000000129 | D005 | Homo sapiens | Male | White | 1 days | Neonate | 39 weeks, 6 days | 39.86 weeks | 2.87 kg | 17 | Congenital Anomaly - Brain | DCD (circulatory death) |
| LMSP0000000034 | D007 | Homo sapiens | Male | African American | 4 years | Child | 15.42 kg | 21 | Trauma - Isolated Head Trauma | DBD (after brain death) | ||
| LMSP0000000135 | D012 | Homo sapiens | Male | White | 2 years | Child | 19.5 kg | 100 | Anoxic/Hypoxic Brain Injury - Drowning | DBD (after brain death) | ||
| LMSP0000000142 | D019 | Homo sapiens | Male | White | 1 days | Neonate | 37 weeks, 3 days | 37.43 weeks | Congenital Anomaly - Brain | DCD (circulatory death) | ||
| LMSP0000000143 | D020 | Homo sapiens | Male | Other | 24 days | Neonate | 41 weeks, 1 days | 44.57 weeks | 4.08 kg | 39 | Anoxic/Hypoxic Brain Injury - SIDS | DBD (after brain death) |
| LMSP0000000035 | D024 | Homo sapiens | Male | Other | 4 months | Infant | 8.62 kg | 94 | Trauma - Auto Accident | DBD (after brain death) | ||
| LMSP0000000152 | D031 | Homo sapiens | Male | White | 7 months | Infant | 40 weeks | 7.26 kg | 7.4 | Anoxic/Hypoxic Brain Injury - SIDS | DBD (after brain death) | |
| LMSP0000000157 | D036 | Homo sapiens | Male | White | 8 years | Child | 34 kg | 89 | Trauma - Isolated Head Trauma | DBD (after brain death) | ||
| LMSP0000000168 | D047 | Homo sapiens | Male | White | 12 days | Neonate | 36 weeks | 37.86 weeks | 3.3 kg | 67 | Stroke or Intracranial Hemorrhage | DBD (after brain death) |
| LMSP0000000173 | D052 | Homo sapiens | Male | African American | 13 months | Child | 9.5 kg | 33 | Trauma - Auto Accident | DBD (after brain death) |
9 of this study's 11 samples were also assayed by 145 other LungMAP studies, across 10 modalities. Click any node to list those studies. The other 2 appear in no other study and are not drawn.