Single cell transcriptomic profiling identifies molecular phenotypes of newborn human lung cells
1University of Rochester Medical Center, *Corresponding author
Description
Frozen single-cell isolates obtained from two newborn human lungs from the LungMAP Human Tissue Core Biorepository, were captured, and library preparation was completed on the Chromium 10X system. Data was analyzed in Seurat, and cellular annotation was performed using the ToppGene functional analysis tool. Transcriptional interrogation of 5500 newborn human lung cells identified distinct clusters representing multiple populations of epithelial, endothelial, fibroblasts, pericytes, smooth muscle, immune cells and their gene signatures. Newborn human lung matrix fibroblasts could be separated into those representative of younger cells (n = 393), or older cells (n = 158). Cells with each molecular profile were spatially resolved within newborn human lung tissue.
Dataset ID
LMEX0000004390
Assay Type
Single-cell RNA-seq
Organism
Human
Stages
Neonate
Cell Count
5499
Technology
10x Genomics Chromium Single Cell Gene Expression
Reference
Bhattacharya, et al. (2024)
| LungMAP ID | Alt ID | Species | Sex | Race | Age | Age Group | GA at Birth | CGA | Weight | Weight Percentile | Cause of Death | Type of Death |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LMSP0000000159 | D038 | Homo sapiens | Female | Unknown or Not Reported | 1 days | Neonate | 39 weeks, 3 days | 39.57 weeks | 2.27 kg | 1.2 | Congenital Anomaly - Brain | DCD (circulatory death) |
| LMSP0000000172 | D051 | Homo sapiens | Female | White | 1 days | Neonate | 31 weeks, 3 days | 31.43 weeks | 1.35 kg | 27 | Congenital Anomaly - Brain | DCD (circulatory death) |
2 of this study's 2 samples were also assayed by 81 other LungMAP studies, across 9 modalities. Click any node to list those studies.