Post-viral flu lung regeneration
Terren K. Niethamer1, Joseph D. Planer1, Michael P. Morley1, Apoorva Babu2, Maria C. Basil1, Edward Cantu2, David B. Frank3, Joshua M. Diamond1, Ana N. Nottingham2, Shanru Li1, Lillian I. Levin1, Su Zhou1, Edward E. Morrisey1*
1Perelman School of Medicine, University of Pennsylvania, 2Penn-Children’s Hospital of Philadelphia Lung Biology Institute, 3Children’s Hospital of Philadelphia, *Corresponding author
Description
The temporal sequencing of mouse lung regeneration after acute viral injury using single-cell RNA-Seq. These data demonstrate that regeneration is asynchronously phased across different cellular compartments. This longitudinal atlas of regeneration has produced a catalogue of new cell states that reflect transient and persistent transcriptional alterations in daughter cells as they transit across axes of differentiation. These new cell states include an injury-induced capillary endothelial cell (iCAP) that arises after injury, persists indefinitely, and shares transcriptional hallmarks with both developing lung endothelium and the endothelial aberrations found in degenerative human lung diseases. This comprehensive atlas of lung regeneration provides a foundational resource to understand the complexity of the cellular and molecular responses to injury, reveals the critical importance of capillary endothelium in maintaining and rebuilding the alveolar niche after injury, and correlates these responses to those found in development and human lung diseases.
LungMAP ID: LMEX0000004402
Organism: mouse
Stages: P4-P36
Assay type: Single-cell RNA-seq
External databases: missing
File Description Size
Mm-Flu-timecourse-adata_compressed.h5ad HDF5 annotated format single-cell data matrix 1.6G