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= Annotated Image
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Fluorescently-labeled secondary antibodies are used to visualize protein localization. Confocal images are pseudo-colored with different colors to represent different proteins in the image. When more than one protein is present in close approximation to each other, the colors will blend into a different color than the original pseudo-colored protein. For example, when red and green are merged, the color will be yellow if the two proteins are in close approximation. If the proteins are at different concentrations or are slightly further away from each other the color will be orange. If the proteins are not within a defined approximation range from each other (depending on magnification) their original colors will appear. The color wheel is provided to helps visualize the merged colors in the fluorescent image.